The participating jurisdictions below have been segmented under Ontario's Regional Tourism Organization (see map below for reference):

RTO1 - Ontario's Southwest
RTO2 - Niagara Region
RTO3 - Hamilton Halton Brant
RTO4 - Huron, Perth, Waterloo and Wellington
RTO5 - Greater Toronto Area
*Currently, within the city of Toronto boundaries, only accommodations, bike shops, bike tours and bike rental locations are eligible to register and become certified as bicycle-friendly within the Ontario By Bike Network.RTO6 - York Durham Headwaters
RTO7 - BruceGreySimcoe
RTO8 - Kawarthas Northumberland
RTO9 - Southeastern Ontario - The Great Waterway
RTO10 - Ottawa and Countryside
RTO11 - Ontario Highlands
RTO12 - Explorers' Edge (Almaguin, Muskoka, Parry Sound & Algonquin Park
RTO13 - North Ontario